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KMID : 0545120170270111932
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Journal of Microbiology and Biotechnology
2017 Volume.27 No. 11 p.1932 ~ p.1941
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In Vitro Effect of DFC-2 on Mycolic Acid Biosynthesis in Mycobacterium tuberculosis
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Kim Su-Kyung
Seo Hoon-Hee
Mahmud Hafij Al
Islam Imtiazul
Kim Yong-Sik
Lyu Ji-Won
Nam Kung-Woo
Lee Byung-Eui
Lee Kee-In
Song Ho-Yeon
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Abstract
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DFC-2, a methyl 5-[2-(dimethylamino)ethoxy]-7,12-dioxo-7,12-dihydrodinaphtho[1,2-b:2’,3’- d]furan-6-carboxylate, is reported to have antitubercular effects against Mycobacterium tuberculosis. At concentrations ranging from 0.19 to 0.39 μg/ml, DFC-2 inhibited both drugsusceptible and ?resistant strains of M. tuberculosis. Microarray analyses were employed to gain insights into the molecular mechanisms of DFC-2’s action in M. tuberculosis. The most affected functional gene category was “lipid biosynthesis,” which is involved in mycolic acid synthesis. The decrease in transcription of genes related to mycolic acid synthesis was confirmed by RT-PCR. Furthermore, we found that DFC-2 triggered a reduction in mycolic acid levels, showing a similar pattern to that of mycolic acid synthesis inhibitor isoniazid. These results may explain how this compound kills mycobacteria efficiently by inhibiting mycolic acid synthesis.
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KEYWORD
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DFC-2, mycolic acid, M. tuberculosis
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